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The BCBC Cell Line Collection is a repository of mouse embryonic stem cell lines for the study of diabetes. As of August 1, 2015, BCBC is no longer in service.
(last updated: Apr 15, 2017)
This source has been archived. We are no longer crawling it or it is no longer updating with new data.
Cell Physical Resource or Software ToolCell Line Name | Parental Cell Line | Targeted Gene | Gene Name | Targeted Allele | Genetic Alteration | Description | ||
---|---|---|---|---|---|---|---|---|
Ptf1aYFP | TL-1 / ptf1a{LCA} | Ptf1a | pancreas specific transcription factor, 1a | Ptf1atm1.1(YFP) | RMCE | This ES cell line contains YFP inserted into a Ptf1aLCA allele by recombinase mediated cassette exchange. These cells may be useful for identifying pancreatic progenitor cells in cultures that have been stimulated to differentiate towards endodermal and pancreatic fates. | Cell line is available | |
Rosa26R26-60-DR5-TA-Cerulean | TL-1 / Rosa26{LCA} clone 5B9 | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | Rosa26tm1(LCA) | RMCE | In these cells Rosa26 gene sequences from -60 to +81 were replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess retinoic acid responsiveness. | ||
Ngn3T2A.nCre | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Foxm1LCA | Not provided | Not provided | Not provided | Not provided | TM | Restricted | ||
Foxa2LCA | Not provided | Not provided | Not provided | Not provided | TM | Restricted | ||
Pax4HA.LCA | Not provided | Not provided | Not provided | Not provided | TM | Restricted | ||
Rosa26228.TOP.CFP | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Rosa263TF.Cherry | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Rosa26TetO.NRSF | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Rosa26Non-EN-CFP-Neo | TL-1 / Rosa26{LCA} clone 5B9 | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | Rosa26tm1.8(Non-EN-CFP) | RMCE | This ES cell line contains CFP (Cerulean) inserted into a Rosa26LCA allele by recombinase mediated cassette exchange. These cells were used to identifythe optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy. | ||
Rosa26mIre1.WT.Cherry | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Pdx1CFP | TL-1 / Pdx1{LCA} clone 2F11 | Pdx1 | pancreatic and duodenal homeobox 1 | Pdx1tm1(LCA) | RMCE | This ES cell line contains cyan fluorescent protein (CFP, Cerulean variant) inserted into a Pdx1LCA allele by recombinase mediated cassette exchange. These cells may be useful for identifying pancreatic progenitor cells in cultures that have been stimulated to differentiate towards endodermal and pancreatic fates. | ||
Ptf1aLCA | TL-1 | Ptf1a | pancreas specific transcription factor, 1a | Ptf1atm1(LCA) | TM | This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 4.1 kb region of this gene (including the proximal promoter and both exons 1 and 2) was replaced with a loxP site, an inverted loxP site, and both positive and negative selectable markers. This enables the use of Recombinase-Mediated Cassette Exchange (RMCE) to easily insert various reporter genes or to make other modifications of the Ptf1a gene locus. | Cell line is available | PMID:18294628 |
Sst.rTTA.LCA | Not provided | Not provided | Not provided | Not provided | TM | Restricted | ||
Rosa26Ngn3.Mafa | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Pdx1Cre | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted | ||
Rosa26-228.3XCNotch.TA.Cerulean | TL-1 / Rosa26{LCA} clone 5B9 | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | Rosa26tm1(LCA) | RMCE | The Rosa26 gene sequences from -60 to -228 were replaced with a repeat of alternating 32-bp C-elements and 31-bp Rbpj-binding sites (i.e.,C-R-C-R-C-R). The 31 base-pair region is derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc; uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh, MCB 16:952, 1996). The 32-bp C-element is from the Ela1 enhancer (Kruse et al., MCB 15:4385, 1995). | Cell line is available | |
Rosa26-228.6XNotch.TA.Cerulean | TL-1 | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | Rosa26tm1(LCA) | RMCE | The binding site for Rbpj, the transcriptional mediator of Notch signaling was inserted into the Rosa 26 locus replacing nucleotides -60 to -228.The complete insert contains the Rbpj cis element in a series of six repeats. Each repeat includes a 31 base-pair region derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc). Uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh et al., MCB 16:952, 1996). | Cell line is available | |
Rosa26Apple.bGsplicepA.Neo | TL-1 | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | Rosa26tm1(LCA) | RMCE | To better quantify protein expression levels via confocal microscopy, flow sorting and western blot analysis this cell line contains a 4kb R26 promoter element, Apple (a red fluorescent protein) with an upstream enhancer element, a splicing beta-globin polyA tail and the neomycin gene as a selection agent. Through Recombinase-Mediated Cassette Exchange this construct was inserted into the Rosa26 locus. | PMID:21324933 | |
Nkx2.2T2A.cCre | Not provided | Not provided | Not provided | Not provided | RMCE | Restricted |
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