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BCBC Protocols are related to primary cell culture, immunostaining, ESC differentiation, cell sorting, and RNA purification. As of August 1, 2015, BCBC is no longer in service.
(last updated: Apr 8, 2017)
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Information Protocols| Protocol Name | Description | Categories | Access |
|---|---|---|---|
| Generate insulin-secreting islet clusters from human iPS | This protocol describes the method for generating insulin-producing islet clusters from human ES cell-like induced pluripotent stem cells derived from human foreskin fibroblast. | ESC directed differentiation | Viewable Request |
| Isolation of Pancreatic Islets from Zebrafish | Purification, Beta cell imaging | Restricted | |
| Manual dissection of E10.5 pancreatic buds | For manual isolation of pancreatic epithelium at early embryonic stages (with little mesenchymal contamination) | Cell sorting, Dissection | Viewable Request |
| Methods for accurate cell tracking in movies of dividing progenitors | Beta cell imaging, Primary culture, Immunostaining, Dissection | Restricted | |
| Pancreas Dissociation for Virus-Mediated Gene Transfer | Primary culture, Dissection | Restricted | |
| Procedure for fractionation and gene expression analysis of juvenile human islet cells. | PCR method, RNA prep. and purification, Cell sorting | Restricted | |
| Strategies for conversion of a DNA aptamer into a real-‐time FRET sensor | None specified | Restricted | |
| Co-culturing endothelial cell lines with embryonic pancreas explants | A method for co-culturing endothelial cell lines with explants of embryonic pancreatic rudiments. | Primary culture | Viewable Request |
| Culturing Different Endothelial Cell Lines | Methods for culturing and splitting four different endothelial cell lines. This information is useful for setting up co-culture experiments with islets or explants from embryonic pancreatic rudiments. | Primary culture | Viewable Request |
| Differentiation of hESCs towards pancreatic like glucose-responsive insulin-secreting cells, in vitro | ESC directed differentiation | Restricted | |
| Directed differentiation of human embryonic stem cell to foregut endoderm | This protocol is for converting human embryonic stem cells to foregut endoderm in the presence of FGF4 (version2 with correction by Martina Johannesson is uploaded on 5/26/09) | ESC directed differentiation | Viewable Request |
| Directed differentiation of mouse embryonic stem cells to foregut endoderm | Optimized protocol for converting mouse embryonic stem cells to foregut endoderm in the presence of FGF4 and Wnt3a | ESC directed differentiation | Viewable |
| Directed differentiation of mouse embryonic stem cells to Sox17+ cells | This protocol is for converting mouse embryonic stem cells to Sox17+ cells using embryoid bodies in the presence of Activin A | ESC directed differentiation | Viewable |
| Islet Transplantation Procedure | Dissection | Restricted | |
| Mouse genotyping | This is the description of a very simple/dependable/cheap mouse genotyping protocol. | PCR method | Viewable Request |
| Non-Human Islet Perifusion | None specified | Restricted | |
| Pancreatic differentiation of hESCs Keller | ESC directed differentiation | Restricted | |
| Preparation and size-enrichment of secreted proteins from mammalian cells | None specified | Restricted | |
| Preparing Islets for Transplant and Calculating IEQs | Dissection | Restricted | |
| Procedure for assessing gene expression in cell subsets from the human fetal pancreas. | RNA prep. and purification | Restricted |
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The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant 2U24DK097771-09.
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