Searching across hundreds of databases
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
The BCBC Mouse Collection provides for the collection and sharing of annotated information on genetically modified mouse strains that are useful for beta cell and/or pancreatic development research. As of August 1, 2015, BCBC is no longer in service.
(last updated: May 8, 2017)
This source has been archived. We are no longer crawling it or it is no longer updating with new data.
Organism Physical Resource or Software Tool| Name | Background | Affected Gene | Genomic Alteration | Transgenic Insertion Type | Notes | Reference | |
|---|---|---|---|---|---|---|---|
| NOD-scid IL2rgamma AB Tg(HLA-DR4) | Not available | Not available | Not available | These NSG-Abo DR4 mice lack expression of the murine Prkdc gene, the X-linked Il2rg gene, and MHC class II, but express the human leukocyte antigen DR4 gene. These mice may be useful for targeting human CD4+ T cells in transplantation studies in the absence of xeno-GVHD.More details are available at http://jaxmice.jax.org/strain/017637.html. | Viewable | ||
| NSG-HLA-A2/HHD | Not available | Not available | Not available | NSG-HLA-A2/HHD mutant mice are immunodeficient and express human HLA class 1 heavy and light chains. This strain may be useful as a human hematopoietic engraftment host that supports the maturation of human T cells with transplantation http://jaxmice.jax.org/strain/014570.html. | Viewable | ||
| riclox | TM | Rictor | 4921505C17Riktm1.1Mgn | Conditional Null | These mice carrya conditional allele for Rictor, a component mTOR complex 2 (mTORC2). Disruption of the Rictor gene attenuates phosphorylation ofSer473 of Akt/PKB, which is important for a normal response to growth factor stimulation acting through the PI3-kinase signaling pathway. | PMID:16962829 | Viewable; Now found at MMRRC |
| ROSAloxP-STOP-LoxP-Myt1 | TM | ROSA26 | Not yet available) | Conditional Activating | Targeted insertion of a LoxP-STOP-LoxP-Myt1 casette into the ROSA26 locus. The presence of Cre recombinase will lead to activation of the 7 zinc finger isoform of Myt1. | Viewable | |
| Pdx1Cre | RMCE | Not available | Not available | Not available | Restricted | ||
| Arx knockout mice | TM | Arx | Arxtm1Pgr | Gene Replacement | These mice were created by insertion of the LacZ gene (with nuclear localization signal) into the Arx locus by homologous recombination in embryonic stem cells. The Arx gene is localized on the X chromosome. Knockout mice die after birth with due to hypoglycemia. Therefore male mice die at day 2 past birth. The colony has to be propagated with female mice. Arx-deficient mice do not produce alpha cells in the islets of Langerhans. | Viewable | |
| Autosomal beta-cell toxigene strain | TM, TG, OTH | interleukin 2 receptor, gamma chain | Il2rgtm1Wjl | Global Null | NOD-Prkdcscid IL2rgnullTg(Ins2-HBEGF)6832Ugfm mice are deficient in mature lymphocytes and NK cells, survive beyond 16 months of age, and even after sublethal irradiation resist lymphoma development. They can be induced to become hypoglycemic when Diphtheria toxin is used. 100% of beta cells will be depleted upon administration of diphtheria toxin. | Viewable Request | |
| Foxa3-Cre | TG | Not available | Not available | Not available | Viewable; Now found at MMRRC | ||
| GhrlCre-eGFP | RMCE | Not available | Not available | Not available | Restricted | ||
| Rosa26mIre1.N906A.Cherry | RMCE | Not available | Not available | Not available | Restricted | ||
| ROSA26Nkx2.2.CFP | RMCE | gene trap ROSA 26, Philippe Soriano | Rosa26tm1 | Cassette Acceptor | In this strain both CFP and myc-tagged Nkx2.2 were inserted into a ROSA26 LCA allele under control of a bidirectional tetO/CMV promoter element. The mouse line will allow us to explore the contribution of Nkx2.2 to progenitor cell population competency by allowing for the manipulation of Nkx2.2 gene expression within the developing pancreas. | Viewable Request | |
| Insulin-Cre | TG | Not available | Not available | Not available | This strain relies on the recombination of floxed DNA in insulin-producing beta cells. | Viewable; Now found at MMRRC | |
| Nkx6.1DsRed2/+ | TM | Nkx6.1 | Nkx6.1tm1 | Global Null | An IRES-DsRed2 cassette was introduced into exon 1 of the Nkx6.1 genomic sequence. The insertion generates a null allele. DsRed2 expression is not detected in these mice. | Viewable Request | |
| NeuroD/lacZ Knock-In | TG | Not available | Not available | Not available | Homozygous phenotype: Postnatal lethal due to neonatal diabetes Pancreas--5% insulin production, no islet formation Gut--no CCK or secretin production Inner ear defects resulting in deafness--no cochlear ganglia and poor vestibular ganglia formation Cerebellar defect resulting in ataxia-- >90% depletion of cerebellar granule cells Hippocampal defects--no dentate gyrus. | Viewable; Now found at MMRRC | |
| Rosa26(mIre1.IG.Cherry) | RMCE | Not available | Not available | Not available | Restricted | ||
| Pdx1-tTA | TM | Pancreatic and duodenal homeobox 1 | Pdx1tmd1Macd | Gene Replacement | Mice homozygous for the targeted mutation fail to develop a pancreas. Heterozygous mice have normal pancreatic development, but have partially impaired glucose tolerance in adulthood. The substitution of the targeted Ipf1/Pdx1 gene with tTAoff inactivates the endogenous allele and places tTAoff expression under the control of the endogenous transcriptional regulatory sequences of the Pdx1 locus. Identical to the endogenous allele, mutant locus expression is detectable in the pancreas and adjacent duodenum but not in other visceral organs or salivary glands. This mutant may be useful to direct tetracycline-regulated expression of responder transgenes in studies of pancreatic endocrine/exocrine development and function and diabetes. This mutant can also be bred with other tetO/TRE strains for pancreas-specific applications. This mutant was originally designed to be mated with mice engineered with a heptameric tetracycline operator (tetO)-controlled bicistronic transgene coding for a normal PDX1 protein and with a beta-galactosidase or EGFP reporter (see BCBC mouse M561). The combined modifications allow normal pancreatic development and function until doxycycline-administration renders the mouse conditionally null of the Pdx1 gene. This configuration for conditional expression of Pdx1is most effective when the transgene locus is homozygous. This allows embryonic developmental arrest at desired stages or cessation of function in adult mice by tetracycline administration. | Viewable Request | |
| Ngn3-Cre | TG | Not available | Not available | Not available | All islet endocrine cells inherit a recombination event, if a segment of DNA is loxP-flanked. Transgene encoding Cre recombinase under the control of a human Neurogenin3 promoter. | Viewable; Now found at MMRRC | |
| biotinTag-Ptf1a | RMCE | pancreas specific transcription factor, 1a | Ptf1atm1 | Cassette Acceptor | Modified allele of Ptf1a with a biotinylation Tag sequence attached to the amino terminus of Ptf1a. | Viewable Request | |
| gkA456V | TM | Glucokinase | Gcktm3Mgn | Global Mutation | This line of mice provides a model for Persistant Hyperinsulinemic Hypoglycemia of Infancy, or PHHI-GK, a rare genetic disease of humans. A gkA456V mutation, originally identified in a human pedigree with PHHI-GK, was introduced into these mice by gene knock-in. These mice may be useful for studies of sustained hypoglycemia. The mutation has been bred into a C57BL/6J strain thereby facilitating direct comparisons to both wild type C57BL/6J animals and to animals with a gkK414E mutation. | Viewable; Now found at MMRRC | |
| Rosa26228.3TF.GFP-Cre | RMCE | Not available | Not available | Not available | Restricted |
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant 2U24DK097771-09.
Email: info_at_ dknet.org
