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The Human Islet Research Network (HIRN) helps to organize and support collaborative research related to the loss of functional beta cell mass in Type 1 Diabetes. The constructs view is a list of constructs used by HIRN researchers. The "Name" column links to the HIRN individual construct pages.
(last updated: Mar 7, 2022)
Molecule Physical Resource or Software ToolName | Description | Insert Name | Vector | Vector Type | Vector Backbone Name | Vendor | |
---|---|---|---|---|---|---|---|
pMDLg.RRE (46b) | Contains Gag & Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev | HIV-1 GAG/POL | viral vectors - pMD | viral vectors | pMD | Addgene plasmid | 12251 |
pLV[Exp]-neo-EF1a-CD19-T2A-eGFP | CD19 is a type of white blood cell found on the surface of B-cells; lentivial expression plasmid (3rd generation) | CD19-T2A-eGFP_2451bp | viral vectors | VectorBuilder | VB161129-1084utf | ||
pCDH-ef1a-INS-DDK-eGFP-PuroR | insulin gene (decreases blood glucose concentration) cloned into mamalian expression plasmid with GFP and puro resistance | System Biosciences | |||||
pCDH-cyto-BirA (Myc-BirA) | cloned the Myc-BirA out of the retrovirus vector pOP-Av and into the lentivirus vector pCDH-EF1α-T2A-puro | System Biosciences | |||||
pCDH-ef1a-MLANA-DDK-eGFP-PuroR | MLANA gene cloned into mamalian expression plasmid with GFP and puro resistance for overexpression | System Biosciences | |||||
pCDH-cyto-Myc BirA | single promoter BirA control plasmid for PTPN22 in the lentiviral expression vector pCDH with the EF1a promoter | System Biosciences | |||||
pMD2-VSVG ENDOfree | VSVG expressing envelope plasmid, also called pMD2.G | VSV G | plasmids - pMD2.G | plasmids | pMD2.G | Addgene plasmid | 11259 |
pRSV.REVB | 3rd generation transfer plasmid | viral vectors | Addgene plasmid | 12253 | |||
pOP-BirA-PTPN22(W)-eGFP | retrovirus plasmid used for PTPN22 BioID containing E. coli biotin ligase BirA with the mutation in PTPN22 at AA 620 (T->W) |
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